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Sagan discusses the story of the Heike crab and artificial selection of crabs resembling samurai warriors, as an opening into a larger discussion of evolution through natural selection (and the pitfalls of intelligent design). Among the topics are the development of life on the Cosmic Calendar and the Cambrian explosion; the function of DNA in growth; genetic replication, repairs, and mutation; the common biochemistry of terrestrial organisms; the creation of the molecules of life in the Miller-Urey experiment; and speculation on alien life (such as life in Jupiter's clouds). In the Cosmos Update ten years later, Sagan remarks on RNA also controlling chemical reactions and reproducing itself and the different roles of comets (potentially carrying organic molecules or causing the Cretaceous–Paleogene extinction event).

Episode 3 delves into how life began on a roiling, violent Earth and explores the story behind Victor Goldschmidt, the man who found the first clues to life's beginnings on our planet. Neil deGrasse Tyson takes viewers on a journey through space and time to witness the tenacity and creativity of life on Earth and the prospects of life throughout the universe.